تأثیر پیش‌تیمار ملاتونین بر برخی فاکتورهای فیزیولوژی در ریزجلبک کلرلا ولگاریس (Chlorella vulgaris) تحت تنش پرتوهای یونیزان

نویسندگان

1 دانشگاه پیام نور

2 دانشگاه کاشان

چکیده

ملاتونین (N- استیل-5-متوکسی‌تریپتامین) دارای نقش مهم و اساسی در بسیاری از فرآیندهای نموی و پاسخ به تنش‌ها، در گیاهان و جلبک‌ها است. در این تحقیق سعی شده تا نقش میانجی‌گری ملاتونین روی میزان رنگیزه‌های فتوسنتزی و پاسخ سیستم دفاع آنتی‌اکسیدان‌های غیرآنزیمی به تنش گاما در سه غلظت شاهد، 100 و 500 میکرومولار در ریزجلبک کلرلا ولگاریس مورد بررسی قرار بگیرد. نتایج نشان داد که جلبک‌هایی که با غلظت 100 میکرومولار ملاتونین پیش‌تیمار شده بودند، دارای رنگیزه‌های فتوسنتزی بیشتر و غشاء پایدارتری در شرایط تنش گاما بودند. بر اساس نتایج این تحقیق، ملاتونین اگرچه می‌تواند تا اندازه‌ای با تقویت سیستم آنتی‌اکسیدان غیرآنزیمی مانند تولید پرولین بیشتر، مقاومت جلبک کلرلا را افزایش دهد ولی این احتمال وجود دارد که این مقاومت بیشتر به سیستم‌های آنتی‌اکسیدانی آنزیمی مرتبط بوده باشد.
 

کلیدواژه‌ها


عنوان مقاله [English]

The effect of Melatonin pretreatment on growth responses and enhancement of the enzyme's antioxidant system in Chlorella vulgaris under stress of ionizing radiation

نویسندگان [English]

  • Payman Aghaie 1
  • Seyed Ali Hosseini Tafreshi 2
  • Ahmad Ramezani moghadam 2
  • Mohammad Amin Toghyani 2
چکیده [English]

Melatonin has important roles in many growth and stress-related responsive processes in plants and algae. In this study, the mediating role of 100 and 500 µM melatonin on photosynthetic pigments and non-enzymatic antioxidant responses of microalga, Chlorella vulgaris to gamma irradiation stress has been studied. The result showed that the alga treated with 100 µM had a stable amount of chlorophyll and a more stability of cell membrane under gamma stress. It was concluded that although melatonin could increase resistance of alga to gamma by strengthen non-enzymatic antioxidant system like more production of proline, however this resistance probably further be related to enzymatic antioxidant system.   
 

کلیدواژه‌ها [English]

  • alga Chlorella vulgaris
  • Melatonin
  • Gamma radiation
  • enzymatic antioxdant system
  • Growth and physiology
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