تأثیر پیش‌تیمار ملاتونین بر برخی فاکتورهای فیزیولوژی در ریزجلبک کلرلا ولگاریس (Chlorella vulgaris) تحت تنش پرتوهای یونیزان

نویسندگان

1 دانشگاه پیام نور

2 دانشگاه کاشان

چکیده

ملاتونین (N- استیل-5-متوکسی‌تریپتامین) دارای نقش مهم و اساسی در بسیاری از فرآیندهای نموی و پاسخ به تنش‌ها، در گیاهان و جلبک‌ها است. در این تحقیق سعی شده تا نقش میانجی‌گری ملاتونین روی میزان رنگیزه‌های فتوسنتزی و پاسخ سیستم دفاع آنتی‌اکسیدان‌های غیرآنزیمی به تنش گاما در سه غلظت شاهد، 100 و 500 میکرومولار در ریزجلبک کلرلا ولگاریس مورد بررسی قرار بگیرد. نتایج نشان داد که جلبک‌هایی که با غلظت 100 میکرومولار ملاتونین پیش‌تیمار شده بودند، دارای رنگیزه‌های فتوسنتزی بیشتر و غشاء پایدارتری در شرایط تنش گاما بودند. بر اساس نتایج این تحقیق، ملاتونین اگرچه می‌تواند تا اندازه‌ای با تقویت سیستم آنتی‌اکسیدان غیرآنزیمی مانند تولید پرولین بیشتر، مقاومت جلبک کلرلا را افزایش دهد ولی این احتمال وجود دارد که این مقاومت بیشتر به سیستم‌های آنتی‌اکسیدانی آنزیمی مرتبط بوده باشد.
 

کلیدواژه‌ها

عنوان مقاله [English]

The effect of Melatonin pretreatment on growth responses and enhancement of the enzyme's antioxidant system in Chlorella vulgaris under stress of ionizing radiation

نویسندگان [English]

  • Payman Aghaie 1
  • Seyed Ali Hosseini Tafreshi 2
  • Ahmad Ramezani moghadam 2
  • Mohammad Amin Toghyani 2
1
2
چکیده [English]

Melatonin has important roles in many growth and stress-related responsive processes in plants and algae. In this study, the mediating role of 100 and 500 µM melatonin on photosynthetic pigments and non-enzymatic antioxidant responses of microalga, Chlorella vulgaris to gamma irradiation stress has been studied. The result showed that the alga treated with 100 µM had a stable amount of chlorophyll and a more stability of cell membrane under gamma stress. It was concluded that although melatonin could increase resistance of alga to gamma by strengthen non-enzymatic antioxidant system like more production of proline, however this resistance probably further be related to enzymatic antioxidant system.   
 

کلیدواژه‌ها [English]

  • alga Chlorella vulgaris
  • Melatonin
  • Gamma radiation
  • enzymatic antioxdant system
  • Growth and physiology

مراجع

[1] F.C. de Abreu, P.N. da Costa, A.M. Brondi, E.J. Pilau, F.C. Gozzo, M.N. Eberlin. Effects of cadmium and copper biosorption on Chlorella vulgaris. Bull Environ Contam Toxicol. 93 (2014) 405–409. [2] K. Ganapathy, K. Chidambaram, R. Janarthanan, R. Ramasamy. Effect of UV-B radiation on growth, photosynthetic activity and metabolic activities of Chlorella vulgaris. J. Microbiol. Biotechnol. 6 (2017) 53–60. [3] S. Hiremath, P. Mathad. Impact of salinity on the physiological and biochemical traits of Chlorella vulgaris. Beijerinck. J. Algal Biomass Utln. 1 (2010) 51–59. [4] S.A. Schaffer. The bioenergetic response of Chlorella vulgaris to alpha radiation. Environ Exp Bot. 25 (1985) 1–6. [5] M. Potts. Mechanisms of desiccation tolerance in cyanobacteria. Eur J Phycol. 34 (1999) 319–328. [6] J-Y. Oh, I.S. Fernando, Y-J. Jeon. Potential applications of radioprotective phytochemicals from marine algae. Algae. 31 (2016) 403–414. [7] N. Zhang, Q. Sun, H. Zhang, Y. Cao, S. Weeda, S. Ren. Roles of melatonin in abiotic stress resistance in plants. J. Exp. Bot. 66 (2014) 647–656. [8] R. Sharif, C. Xie, H. Zhang, M. Arnao, M. Ali, Q. Ali. Melatonin and Its Effects on Plant Systems. Molecules. 23 (2018) 23–52. [9] Q-H. Han, B. Huang, C-B. Ding, Z-W. Zhang, Y-E. Chen, C. Hu. Effects of melatonin on anti-oxidative systems and photosystem II in cold-stressed rice seedlings. Front. Plant Sci. 8 (2017) 785. [10] D, Ghosal, M.V. Omelchenko, E.K. Gaidamakova, V.Y. Matrosova, A. Vasilenko, A. Venkateswaran. How radiation kills cells: survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress. FEMS Microbiol Rev. 29 (2005) 361–75. [11] J-i. Choi, M. Yoon, S. Lim, G.H. Kim, H. Park. Effect of gamma irradiation on physiological and proteomic changes of Arctic Zygnema sp. (Chlorophyta, Zygnematales). Phycologia. 54 (2015) 333–341. [12] D. Billi, E.I. Friedmann, K.G. Hofer, M.G. Caiola, R. Ocampo-Friedmann. Ionizing-radiation resistance in the desiccation-tolerant cyanobacterium Chroococcidiopsis. Appl. Environ. Microbiol. 66 (2000) 1489–1492. [13] H.K. Lichtenthaler, A.R. Wellburn. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Portland Press LTD. 5 (1983) 67–79. [14] C. Liu, Y. Liu, K. Guo, D. Fan, G. Li, Y. Zheng Effect of drought on pigments, osmotic adjustment and antioxidant enzymes in six woody plant species in karst habitats of southwestern China. ENVIRON EXP BOT. 71 (2011) 174–183. [15] R.L. Heath, L. Packer. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125 (1968) 189–198. [16] L. Bates, R. Waldren, I. Teare. Rapid determination of free proline for water-stress studies. Plant and Soil. 39 (1973) 205–207. [17] M. Ashraf, P. Harris. Photosynthesis under stressful environments: an overview. Photosynthetica. 51 (2013) 163–190. [18] S. Perveen, M. Shahbaz, M. Ashraf. Regulation in gas exchange and quantum yield of photosystem II (PSII) in salt-stressed and non-stressed wheat plants raised from seed treated with triacontanol. Pak J Bot. 42 (2010) 3073–3081. [19] S. Baccouch, A. Chaoui, E.l. Ferjani, E. Nickel. Toxicity induces oxidative damage in Zea mays roots. J Plant Nutr. 24 (2001) 1085–1097. [20] H. Turk, S. Erdal, M. Genisel, O. Atici, Y. Demir, D. Yanmis. The regulatory effect of melatonin onphysiological, biochemical and molecular parameters in cold-stressed wheat seedlings. Plant Growth Regul. 74 (2014) 139–152. [21] Z. Hu, J. Fan, Y. Xie, E. Amombo, A. Liu, M.M. Gitau. Comparative photosynthetic and metabolic analyses reveal mechanism of improved cold stress tolerance in Bermudagrass by exogenous melatonin. Plant Physiol Biochem.100 (2016) 94–104. [22] J. Liu, W. Wang, L. Wang, Y. Sun. Exogenous melatonin improves seedling health index and drought tolerance in tomato. Plant Growth Regul. 77 (2015) 317–326. [23] N. Zhang, H. J. Zhang, B. Zhao, Q.Q. Sun, Y.Y. Cao, R. Li. The RNA-seq approach to discriminate gene expression profiles in response to melatonin on cucumber lateral root formation. J. Pineal Res. 56 (2014) 39–50. [24] Z. Elmi Anaraki, M. Shariati, S.A. Hosseini Tafreshi. Transient silencing of phytoene desaturase reveals critical roles on plant response to salinity stress. Acta Physiol Planta. 39 (2017) 161–178.

فایل ها

هم رسانی

ارجاع به این مقاله

آمار