Biological Dose Assessment by Chemical Induction of Premature Chromosome Condensation

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Abstract

Dose assessment of radiation victims is a key element in medical management of radiation accidents. At high radiation doses (> 6 Gy), dicentric assay lose its efficiency and premature chromosome condensation (PCC) assay was proposed to overcome the restrictions of dicentric assay. It was shown that PCC ring is more suitable and simpler than dicentric for biodosimetry in high radiation doses. In this study, we established standard dose response curve using chemical PCC ring assay on human peripheral blood lymphocytes for X-ray in the dose-interval of 0–10 Gy. The chemical PCC assay performed on Peripheral blood lymphocyte of four healthy donors after irradiation with different doses of X-rays (0-10 Gy) and the IAEA recommendations for production of an in vitro dose response curve were followed. The dose response curve was fitted based on the linear quadratic model and practicable for high radiation doses. Chemical PCC ring assay is applicable with no need to any special equipment even after supra lethal radiation doses.

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References

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