Retrospective assessment of radiation dose by Fluorescence In Situ Hybridization and evaluation of stable chromosomal aberrations

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10.22052/8.1.5

Abstract

Estimation of absorbed dose for radiation workers or person involved in different radiological accident is the aim of biodosimetry. Cytogenetic methods are the most current and applicable biodosimetry tools. In chronic or protracted exposure, fluorescence in situ hybridization (FISH), stable chromosomal aberration is used for estimation of absorbed dose. For precise estimation of absorbed dose, every biodosimetry department should prepare standard dose response curve for different dose and dose rates. In this study, after sampling of blood from two healthy males, blood samples irradiated with X- ray of linear accelerator (0.5-2 Gy) and after separation of their lymphocytes, culturing and metaphase spread were prepared. Fluorescence in situ hybridization painting was performed and stable chromosomal aberration was recorded. Dose response curve was prepared for stable chromosomal aberration for different X-ray doses which can use for retrospective biodosimetry in occupational and accidental situations.

Keywords

References

[1] United States Government US Army. Medical consequences of radiological and nuclear weapons, (2013). [4] ISO 19238. Radiation protection: Performance criteria for service laboratories performing biological dosimetry by cytogenetics (2014). [5] IAEA. Cytogenetic Dosimetry: Application in preparedness for and response to Radiation Emergencies. Emergency Preparedness and Response Series (2011). [6] M.L. Camparoto, A.T. Ramalho, A.T. Natarajan, M.P. Curado and E.T. Sakamoto-Hojo. Translocation analysis by the FISH-painting method for retrospective dose reconstruction in individuals exposed to ionizing radiation 10 years after exposure. Mutat Res. 530(1-2) (2003) 1–7. [7] Q.J. Liu, X. Lu and X.T. Zhao. Assessment of retrospective dose estimation, with fluorescence in situ hybridization (FISH), of six victims previously exposed to accidental ionizing radiation. Mutat Res Genet Toxicol Environ Mutagen. 759 (2014) 1–8. [8] L. Stronati, M. Durante, G. Gensabella, G. Gialanella, G.F. Gross ,M. Pugliese, P. Scampoli, A. Sgura, A. Testa and C. Tanzarella. Calibration curves for biological dosimetry by fluorescence in situ hybridisation. Radiat Prot Dosimetry. 94(4) (2001) 335–345. [9] J.F. Barquinero, S. Cigarrán, M.R. Caballín, H. Braselmann, M. Ribas, J. Egozcue and L. Barrios. Comparison of X-ray dose-response curves obtained by chromosome painting using conventional and PAINT nomenclatures. Int J Radiat Biol. 75(12) (1999) 1557–1566. [10] A.V. Sevan'kaev, I.K. Khvostunov, G.F. Mikhailova, E.V. Golub, O.I. Potetnya, N.N. Shepel, V.Y. Nugis and N.M. Nadejina. Novel data set for retrospective biodosimetry using both conventional and FISH chromosome analysis after high accidental overexposure. Appl Radiat Isot. 52(5) (2000) 1149–1152. [11] J.F. Barquinero, C. Beinke and M. Borràs. RENEB biodosimetry intercomparison analyzing translocations by FISH. Int J Radiat Biol. 93(1) (2017) 30–35. [12] A.A. Edwards, M. Szluinska and D.C. Lloyd. Reconstruction of doses from ionizing radiation using fluorescence in situ hybridization techniques. Br J Radiol. 80(1) (2007) S63–67. [13] E. Grégoire, L. Roy, V. Buard and M. Delbos. Twenty years of FISH-based translocation analysis for retrospective ionizing radiation biodosimetry. Int J Radiat Biol. 94(3) (2018) 248–258. [14] M.J. Ramsey, D.H. Moore , J.F. Briner, D.A. Lee , L. Olsen, J.R. Senft and J.D. Tucker. The effects of age and lifestyl.e factors on the accumulation of cytogenetic damage as measured by chromosome painting. Mutat Res. 338(1-6) (1995) 95–106. [15] I. Sorokine-Durm, C. Whitehouse and A.A. Edwards. The variability of translocation yields amongst control populations. Radiat Prot Dosimetry 88(1) (2000) 93–99. [16] J.N. Lucas and W. Deng. Our views on issues in radiation biodosimetry based on chromosome aberrations measured by FISH. Radiat Prot Dosimetry. 88(1) (2000) 77–86. [17] Y. Suto, M. Akiyama, T. Noda and M. Hirai. Construction of a cytogenetic dose-response curve for low-dose range gamma-irradiation in human peripheral blood lymphocytes using three-color FISH. Mutat Res Genet Toxicol Environ Mutagen. 794 (2015) 32–38. [18] C.A. Whitehouse, A.A. Edwards, E.J. Tawn, G. Stephan, U. Oestreicher and JE Moquet. Translocation yields in peripheral blood lymphocytes from control populations. Int J Radiat Biol. 81(2) (2005) 139–145. [19] Tucker JD. Low-dose ionizing radiation and chromosome translocations: a review of the major considerations for human biological dosimetry. Mutat Res. 659(3) (2008) 211–220. [20] P.C. Van Diemen, D. Maasdam, S. Vermeulen, F. Darroudi and A.T. Natarajan. Influence of smoking habits on the frequencies of structural and numerical chromosomal aberrations in human peripheral blood lymphocytes using the fluorescence in situ hybridization (FISH) technique. Mutagenesis. 10(6) (1995) 487–495. [21] J.D. Tucker and D.H. Moore. The importance of age and smoking in evaluating adverse cytogenetic effects of exposure to environmental agents. Environ Health Perspect. 104(Suppl 3) (1996) 489–492.

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