Comparison of the effects of ultraviolet and gamma rays on arsenic tolerance in the bioleaching process

Authors

10.22052/5.4.1

Abstract

One of the factors which can kill or decrease the viability of bioleaching bacteria is the creation of toxicity due to the process of metal release from mine stone. This toxicity is partly due to the presence of arsenic in mine stone. In the present study mutagenesis method was used to improve the bioleaching process performance by using UV and gamma rays. The influence of radiations (UV& gamma rays) on growth and death of Thiobacillus ferrooxidans bacteria were investigated at different time exposures.  Afterwards, a defined concentration of arsenic salt (NaAsO5) was added to the remaining bacteria until mutant bacteria with arsenic resistance were separated. The results showed that irradiation time of 180 second and 120 minute of gamma and UV rays, causes the number of bacteria to increase by 20% and 45%, respectively.
 

Keywords


[2] M. Caudill. The Effects of Arsenic on Thiobacillus ferrooxidans (2003) Thesis (MSc, Columbia University, Earth and Environmental Engineering Department, Columbia). [3] X. Xie. Bioleaching of Arsenic-Rich Gold Concentrates by Bacterial Flora before and after Mutation. BioMed Research International, Vol. 2013 (2013) Article ID 969135, 10 pages.‌ [4] P.I. Harvey and F.K. Crundwell. The Effect of As(III) on the Growth of Thiobacillus ferrooxidans in an Electrolytic Cell under Controlled Redox Potentials. Minerals Engineering, 9 (1996) 1059–1068. [5] A. Jensen and C. Webb. Ferrous Sulphate Oxidation Using Thiobacillus ferrooxidans a Review. Process Biochemistry, 3(3) (1995) 225–236. [6] R. West, G. Stephens and J. Cilliers. Zeta Potential of Silver Absorbing Thiobacillus ferrooxidans. Mineral Engineering, 12(6) (2005) 189–194. [7] C. Meng, X. Shi, H. Lin, J. Chen, Y. Guo. UV Induced Mutations in Acidianus brierleyi Growing in a Continuous Stirred Tank Reactor Generated a Strain with Improved Bioleaching Capabilities. Enzyme and Microbial Technology, 40 (2007) 1136–1140. [8] K. Takatsugi, K. Sasaki and T. Hirajima. Mechanism of the Enhancement of Bioleaching of Copper from Enargite by Thermophilic Iron Oxidizing Archaea with the Concomitant Precipitation of Arsenic. Hydrometallurgy, 109 (2011) 90–96.